This study determined the factors affecting motivation and job satisfaction of non-management academic staff of universities in South-South geopolitical zone of Nigeria. It employed an expost-facto research design. Three research questions and two hypotheses were raised for the study. A sample of four hundred and fifty non-management academic staff was administered the instrument for data collection. The instrument was vetted by three experts in the field of education, and face and content validity was established. The reliability coefficient of 0.82 was computed using Cronbach Alpha formula to measure the internal consistency of the questionnaire items. The three research questions were answered using mean and standard deviation, while the two hypotheses were tested using the z-test statistics at 0.05 level of significance. Results revealed that there is no significant difference between male and female non-management academic staff motivation and job satisfaction. It was also found that highly motivated non-management academic staffs perform their job better than poorly motivated staff. It was recommended that university authorities and the government should pay increasing attention to the motivation of non-management academic staff in order to boost their job performance and satisfaction thereby enhancing high productivity.