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ERIC Number: EJ1069983
Record Type: Journal
Publication Date: 2014-Dec
Pages: 6
Abstractor: As Provided
Reference Count: 14
ISBN: N/A
ISSN: ISSN-1539-2422
Infusing Bioinformatics and Research-Like Experience into a Molecular Biology Laboratory Course
Nogaj, Luiza A.
Bioscene: Journal of College Biology Teaching, v40 n2 p3-8 Dec 2014
A nine-week laboratory project designed for a sophomore level molecular biology course is described. Small groups of students (3-4 per group) choose a tumor suppressor gene (TSG) or an oncogene for this project. Each group researches the role of their TSG/oncogene from primary literature articles and uses bioinformatics engines to find the gene and promoter sequence of their TSG/oncogene. Based on the promoter sequences, students design appropriate primers for the PCR amplification and cloning of the promoter of the gene of interest and perform a diagnostic digest to confirm the results. Finally, each student writes an individual report about his or her findings and results and each group presents the results to the class. This laboratory sequence teaches students how to read primary literature, use common bioinformatics engines, clone a DNA sequence, and present the results in an oral and written format.
Association of College and Biology Educators. Web site: http://acube.org
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Institutes of Health (DHHS)
Authoring Institution: N/A
IES Grant or Contract Numbers: 5T36GM078011-05