Information and Communication Technology (ICT) integration in teacher education and teaching practices of teachers is a complicated and challenging issue. As far as pre-service teachers are concerned, this becomes critical because they need to be equipped with the competencies for their future teaching practices. The objective of this study is to identify pre-service teachers' perceptions of ICT integration in teacher education and its association with their teaching practices. A stratified two-stage probability sampling design was used. Firstly, three Turkish state universities with the highest and lowest number of the pre-service teachers were chosen. Secondly, pre-service teachers from the subject domains of Turkish language, social sciences, elementary education mathematics and science were selected. A qualitative method was used in this study. Data were collected from 782 pre-service teachers from open ended questions in a survey and interviews were conducted with 15 participants. Qualitative data were analyzed with thematic coding. The results identified the specific conditions of ICT integration in teacher education and pre-service teachers' perceptions of ICT integration and the associations with their teaching practices.