Measurement invariance of the five-factor "Servant Leadership Questionnaire" between female and male K-12 principals was tested using multi-group confirmatory factor analysis. A sample of 956 principals (56.9% were females and 43.1% were males) was analysed in this study. The hierarchical multi-step measurement invariance test supported the measurement invariance of the five-factor model across gender. Latent factor means were compared between females and males when measurement invariance was established. Results showed that females were significantly higher than males on emotional healing, wisdom, persuasive mapping and organisational stewardship, and they were not statistically different on altruistic calling.