Objective: To explore the anaerobic power and surface EMG (sEMG) of quardrocep muscle in lower extremities after single vibration training intervention. Methods: 8 excellent male long jumpers voluntarily participated in this study. Four intervention modes were devised, including high frequency high amplitude (HFHA,30Hz,6mm), low frequency low amplitude (LFLA,15Hz,3mm), high frequency low amplitude (HFLA,30Hz,3mm), and non-vibration (CON). All subjects received a knee extensor maximal voluntary contraction (MVC) test before the vibration. After the test, a 5×1 min. vibration would be carried out and the subjects were asked to pedal. Results: Peak power and average power output of the four vibration treatment methods did not reach obvious level. There was no obvious difference in root-mean-square (RMS) EMG of the dominant leg among the four treatments. The RMS EMG of the non-dominant leg of HFLA method during peak power output was much higher than that of HFHA and CON. The quotient between root-mean-square and peak power of the four groups did not reach significant level. Conclusion: Single vibration could not improve the anaerobic performance of long jumpers and HFLA might decrease the work efficiency of the non-dominant leg muscle of excellent long jumpers participating anaerobic test.