The study assessed teaching performance of 222 student-teachers from the Faculty of Education, Ekiti State University, posted to various secondary schools in Ekiti State for a six-week teaching practice during 2010/2011 academic session. The sample included 119 males, 103 females, 78 (300-Level) and 144 (400-Level) students. Data were collected using Teaching Performance Assessment Form designed by the Faculty of Education, Ekiti State University and analysed using frequency counts, percentages, item-total correlations and t-test comparison, tested at 0.05 level of significance. Results showed satisfactory teaching performance of the student-teachers while gender and previous teaching experience had no significant influence on teaching performance of the student-teachers. It was recommended that supervisors (of teaching practice/raters) should be thoroughly trained on the use of assessment instrument for reliable measurement of student-teachers' teaching performance.