The present study enlightens the impact of Learner-Oriented-Co-operative Learning in enriching knowledge in Environmental Education at Higher Education. To achieve the expected competency in Biodiversity, various approaches were adopted in the class room transaction which were not fruitful. Hence the researcher practiced the Learner-Oriented-Co operative Learning in the classroom transaction. Single group Experimental method was adopted in the study. Qualitative and quantitative approaches were adopted in the study. Twenty five students studying in Master of Commerce at Bharathiar University, Coimbatore, Tamilnadu were considered as sample for the study. Achievement test was considered as tool for the study. Resarcher's self-made tool was used for pretest and post test. After administering pre test, treatment was given through Learner-Oriented-Co-operative Learning to find out the effectiveness of it. Descriptive statistics was applied for the study. Learner-oriented-Cooperative Learning is more effective than traditional method in Learning Biodiversity in Environmental Education. The fruitful method can be implemented in some other Universities and colleges for other subjects also.