The purpose of this study was to explore how satisfied prospective science teachers are with their department (academic staff and administration) at different Faculties of Education in Turkey. For this purpose, Prospective Science Teachers Satisfaction Questionnaire (PSTSQ) was developed by considering related literature. PSTSQ consists of two parts and seven dimensions, namely General Satisfaction, Administration, Curriculum, Academic Staff, Facilities, Skills Promoted by Courses and Laboratory and its Facilities, respectively. In order to explore prospective teachers' satisfaction level, PSTSQ was administered to 410 fourth-year students who enrolled in science education programs at six different Faculties of Education. The participants of the study were asked to respond to the 82 items on a 5 point Likert-scale (1 = strongly disagree and 5 = strongly agree). The reliability analysis indicated that Cronbach's alpha reliability coefficient (a) of the instrument was 0.89. For the purpose of the present study, only the administration and academic staff dimensions of the questionnaire were used. The participants reported that they were dissatisfied with some aspects of their department, but there was no significant mean difference between male and female students. On the contrary, significant mean differences among students from different universities in terms of their satisfaction with their departments were found.