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ERIC Number: EJ1062350
Record Type: Journal
Publication Date: 2015-May
Pages: 4
Abstractor: As Provided
Reference Count: 12
ISSN: ISSN-0021-9584
Fighting Tuberculosis in an Undergraduate Laboratory: Synthesizing, Evaluating and Analyzing Inhibitors
Daniels, David; Berkes, Charlotte; Nekoie, Arjan; Franco, Jimmy
Journal of Chemical Education, v92 n5 p928-931 May 2015
A drug discovery project has been successfully implemented in a first-year general, organic, and biochemistry (GOB) health science course and second-year organic undergraduate chemistry course. This project allows students to apply the fundamental principles of chemistry and biology to a problem of medical significance, practice basic laboratory skills, and understand the interdisciplinary aspect of the drug discovery platform. Students collectively synthesize a small library of antituberculosis compounds and subsequently screen them using a Kirby-Bauer disc diffusion assay for inhibitory activity against "Mycobacterium smegmatis", a known safe surrogate of "Mycobacterium" tuberculosis, the causative agent of tuberculosis. The last unit of the project involves students using PyMOL, a free computer program, to examine a cocrystal structure of the activated inhibitor-enzyme complex, allowing students to identify the molecular interactions responsible for the binding affinity.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: Teachers
Language: English
Sponsor: N/A
Authoring Institution: N/A