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ERIC Number: EJ1062308
Record Type: Journal
Publication Date: 2015-Apr
Pages: 5
Abstractor: As Provided
Reference Count: 18
ISBN: N/A
ISSN: ISSN-0021-9584
Undergraduate Laboratory Module for Implementing ELISA on the High Performance Microfluidic Platform
Giri, Basant; Peesara, Ravichander R.; Yanagisawa, Naoki; Dutta, Debashis
Journal of Chemical Education, v92 n4 p728-732 Apr 2015
Implementing enzyme-linked immunosorbent assays (ELISA) in microchannels offers several advantages over its traditional microtiter plate-based format, including a reduced sample volume requirement, shorter incubation period, and greater sensitivity. Moreover, microfluidic ELISA platforms are inexpensive to fabricate and allow integration of analytical procedures, such as sample preconcentration, that further enhance the performance of the immunoassay. In view of the scientific potential of microfluidic ELISAs, inclusion of this technique into an undergraduate curriculum is valuable in preparing the next generation of scientists and engineers. Here, an experimental module is presented for this immunoassay method that can be completed in an undergraduate laboratory setting within two 3-h periods (including all incubation and data analyses procedures) using only a microliter of sample and reagents per assay. In addition to acquainting students with the microfluidic technology, the reported module provides training in quantitating ELISAs using the kinetic format of the assay. Furthermore, it offers a useful educational tool for introducing undergraduates to basic image analysis techniques, as well as signal-to-noise ratio and limit of detection calculations that are valuable in characterizing any analytical method.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Science Foundation; National Institutes of Health (DHHS)
Authoring Institution: N/A
IES Grant or Contract Numbers: DBI 0964211|1R15AG045755-01A1