This research project represents the Status of Biodiversity and Its Conservation of Kobadak River basin of Maheshpur Upazila. The study was designed to develop a set of information about the present condition of biodiversity of the study area. Both primary and secondary data have been used to fulfill the survey successfully. Primary data have been collected from focused group discussion, key informant interview and secondary data have been collected from journals, books, websites etc. and maps have been collected from websites. The significant importance of this survey is to conduct with a view to expand our knowledge on biodiversity conditions of Kapotakkho River at Maheshpur Upazila, Jhenaidah. Collected data from the study area indicates that the condition of biodiversity of the area is gradually deteriorating. This study also shows that many species is under threat of extinction. The main causes include pollution and contamination, siltation on riverbed, over-exploitation, and lack of awareness and also include lack of government initiatives and effective steps from NGOs. So, government initiative must be taken to protect biodiversity and maintain environmental balance. This study would help understand the present situation of the study area.