There has been little research on undergraduate reproductive anatomy education. This pilot study explores knowledge of anatomical reproductive anatomy among university students in a lower division and upper division health course. Using a Qualtrics survey program, a convenience sample of 120 lower division and 157 upper division students for a total population of 227 from one California public university were given a demographic survey prior to testing knowledge of female and male reproductive anatomy. A prediction equation validated the null hypothesis. Survey results supported the potential differentiation in what is taught in the lower and upper division classes and indicated the need for further research to assist the professor in determining the depth and breadth of information to be included in curriculum.