NotesFAQContact Us
Search Tips
Peer reviewed Peer reviewed
Direct linkDirect link
ERIC Number: EJ1050716
Record Type: Journal
Publication Date: 2015-Jan
Pages: 4
Abstractor: ERIC
ISSN: ISSN-0021-9584
Normal and Reversed-Phase Thin Layer Chromatography of Green Leaf Extracts
Sjursnes, Birte Johanne; Kvittingen, Lise; Schmid, Rudolf
Journal of Chemical Education, v92 n1 p193-196 Jan 2015
Introductory experiments of chromatography are often conducted by separating colored samples, such as inks, dyes, and plant extracts, using filter paper, chalk, or thin layer chromatography (TLC) plates with various solvent systems. Many simple experiments have been reported. The relationship between normal chromatography and reversed-phase chromatography is, however, seldom illustrated with experiments. Where such experiments exist, they often utilize column chromatography and, particularly, HPLC, although TLC systems and paper chromatography have also been reported. In this article, the authors report how normal and reversed-phase TLC can be illustrated by separating extracts of spinach ("Spinacia oleracea") and rocket salad/ruccola ("Eruca sativa"). The novelty of this experiment is that (i) the chromatographic principle is different, that is, TLC (adsorption chromatography) compared to paper chromatography (partition chromatography); (ii) the extract material (ruccola instead of dandelions) is more available but still contains sufficient amount of pheophytin; (iii) the system has been extensively optimized with respect to resolution; and (iv) the quantity of material (TLC plates and solvent) has been reduced substantially, making the experiment inexpensive, time-efficient (elution time reduced from 40 to 8 min), and more in line with health and safety regulations.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A