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ERIC Number: EJ1050698
Record Type: Journal
Publication Date: 2015-Jan
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
"Supermarket Column Chromatography of Leaf Pigments" Revisited: Simple and Ecofriendly Separation of Plant Carotenoids, Chlorophylls, and Flavonoids from Green and Red Leaves
Dias, Alice M.; Ferreira, Maria La Salete
Journal of Chemical Education, v92 n1 p189-192 Jan 2015
A simple and ecofriendly procedure was developed in order to prepare extracts from red and green leaves. This procedure enables the separation of yellow, green, and red band pigments and optimizes the previously reported baking soda "supermarket column". The same extract also led to a novel and colorful potato starch column, which can efficiently separate ß-carotene, xanthophylls, chlorophylls "a" and "b", and flavonoids. Thus, two simple and attractive "supermarket columns" are presented that may be implemented either in university or high-school practical classes. The challenges associated with separation of these pigments, which have diverse physical and chemical properties, will improve the expected learning outcomes of these activities.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Secondary Education; High Schools; Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A