ERIC Number: EJ1048868
Record Type: Journal
Publication Date: 2015-Jan
Abstractor: As Provided
Reference Count: N/A
Transcriptional Analysis of a Whole-Body Form of Long-Term Habituation in "Aplysia Californica"
Holmes, Geraldine; Herdegen, Samantha; Schuon, Jonathan; Cyriac, Ashly; Lass, Jamie; Conte, Catherine; Calin-Jageman, Irina E.; Calin-Jageman, Robert J.
Learning & Memory, v22 n1 p11-23 Jan 2015
Habituation is the simplest form of learning, but we know little about the transcriptional mechanisms that encode long-term habituation memory. A key obstacle is that habituation is relatively stimulus-specific and is thus encoded in small sets of neurons, providing poor signal/noise ratios for transcriptional analysis. To overcome this obstacle, we have developed a protocol for producing whole-body long-term habituation of the siphon-withdrawal reflex (SWR) of "Aplysia californica." Specifically, we constructed a computer-controlled brushing apparatus to apply low-intensity tactile stimulation over the entire dorsal surface of "Aplysia" at regular intervals. We found that 3 d of training (10 rounds of stimulation/day; each round = 15 min brushing at a 10-sec ISI; 15-min rest between rounds) produces habituation with several characteristics favorable for mechanistic investigation. First, habituation is widespread, with SWR durations reduced whether the reflex is evoked by tactile stimulation to the head, tail, or the siphon. Second, long-term habituation is sensitive to the pattern of training, occurring only when brushing sessions are spaced out over 3 d rather than massed into a single session. Using a custom-designed microarray and quantitative PCR, we show that long-term habituation produces long-term up-regulation of an apparent "Aplysia" homolog of cornichon, a protein important for glutamate receptor trafficking. Our training paradigm provides a promising starting point for characterizing the transcriptional mechanisms of long-term habituation memory.
Descriptors: Learning Processes, Habituation, Tactual Perception, Memory, Stimulation, Statistical Analysis, Biochemistry, Genetics, Entomology
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Publication Type: Journal Articles; Reports - Research
Education Level: N/A
Authoring Institution: N/A