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ERIC Number: EJ1048350
Record Type: Journal
Publication Date: 2014-Dec
Pages: 3
Abstractor: As Provided
Reference Count: 12
ISBN: N/A
ISSN: ISSN-0021-9584
Kinase Activity Studied in Living Cells Using an Immunoassay
Bavec, Aljos?a
Journal of Chemical Education, v91 n12 p2171-2173 Dec 2014
This laboratory exercise demonstrates the use of an immunoassay for studying kinase enzyme activity in living cells. The advantage over the classical method, in which students have to isolate the enzyme from cell material and measure its activity in vitro, is that enzyme activity is modulated and measured in living cells, providing a more realistic picture of cell metabolism. With this experiment, students learn not only how to study protein kinases but also how to study the MAP kinase signaling pathway, how to set up their own experiments, and how to study the effects of pharmacologically important ligands on signaling in a particular metabolic pathway. Signaling with protein kinases, as described here, is a good example of how to study molecular mechanisms of signaling in general.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A