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ERIC Number: EJ1046295
Record Type: Journal
Publication Date: 2014-Nov
Pages: 5
Abstractor: As Provided
ISSN: ISSN-0021-9584
Development and Validation of a Fast Procedure to Analyze Amoxicillin in River Waters by Direct-Injection LC-MS/MS
Homem, Vera; Alves, Arminda; Santos, Lu´cia
Journal of Chemical Education, v91 n11 p1961-1965 Nov 2014
A laboratory application with a strong component in analytical chemistry was designed for undergraduate students, in order to introduce a current problem in the environmental science field, the water contamination by antibiotics. Therefore, a simple and rapid method based on direct injection and high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed and optimized for the determination of amoxicillin in river water. Students learned the main optimization steps for the improvement of the LC-MS/MS methodology and the correct procedure to validate an analytical method (draw a calibration curve, determine detection and quantification limits, precision, and accuracy). This laboratory experiment was successfully applied by students and enables the analysis of a large number of samples in a short period of time because of the short run time (3 min).
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A