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ERIC Number: EJ1045439
Record Type: Journal
Publication Date: 2014
Pages: 6
Abstractor: As Provided
Reference Count: N/A
ISSN: ISSN-1470-8175
Isolation and Genetic Analysis of an Environmental Bacteriophage: A 10-Session Laboratory Series in Molecular Virology
Williamson, Ryan P.; Barker, Brent T.; Drammeh, Hamidou; Scott, Jefferson; Lin, Joseph
Biochemistry and Molecular Biology Education, v42 n6 p480-485 Nov-Dec 2014
Bacterial viruses, otherwise known as bacteriophage (or phage), are some of the most abundant viruses found in the environment. They can be easily isolated from water or soil and are ideal for use in laboratory classrooms due to their ease of culture and inherent safety. Here, we describe a series of 10 laboratory exercises where students collect, isolate, and purify the genome of an environmental phage. Once the genome has been extracted, students then clone a fragment of their isolated phage genome into a plasmid and analyze its sequence to identify the phage in their original isolate. These exercises have been carefully designed to apply foundational concepts that will expose students to basic skills in microbiology, molecular biology, and bioinformatics.
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A