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ERIC Number: EJ1036386
Record Type: Journal
Publication Date: 2014-Aug
Pages: 4
Abstractor: As Provided
Reference Count: 20
ISBN: N/A
ISSN: ISSN-0021-9584
Examination and Manipulation of Protein Surface Charge in Solution with Electrospray Ionization Mass Spectrometry
Gross, Deborah S.; Van Ryswyk, Hal
Journal of Chemical Education, v91 n8 p1240-1243 Aug 2014
Electrospray ionization mass spectrometry (ESI-MS) is a powerful tool for examining the charge of proteins in solution. The charge can be manipulated through choice of solvent and pH. Furthermore, solution-accessible, protonated lysine side chains can be specifically tagged with 18-crown-6 ether to form noncovalent adducts. Chemical derivatization of a protein in this fashion followed by "soft" ionization can provide a wealth of specific information regarding side chain identity, location, and surface chemistry. A variety of proteins are examined with ESI-MS under a range of conditions in this self-guided experiment, and the results are compared with those predicted from structural information available in the protein data bank.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: National Science Foundation
Authoring Institution: N/A
IES Grant or Contract Numbers: 0077540|0922481