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ERIC Number: EJ1030300
Record Type: Journal
Publication Date: 2014-Jan
Pages: 7
Abstractor: As Provided
Reference Count: N/A
ISSN: ISSN-0021-9584
Illustrating the Effect of pH on Enzyme Activity Using Gibbs Energy Profiles
Bearne, Stephen L.
Journal of Chemical Education, v91 n1 p84-90 Jan 2014
Gibbs energy profiles provide students with a visual representation of the energy changes that occur during enzyme catalysis, making such profiles useful as teaching and learning tools. Traditional kinetic topics, such as the effect of pH on enzyme activity, are often not discussed in terms of Gibbs energy profiles. Herein, the symbolism of Gibbs energy profiles is utilized to derive expressions for the changes in Gibbs energy associated with the apparent enzyme kinetic parameters K[subscript S][superscript app], k[subscript cat][superscript app], and (k[subscript cat]/K[subscript M])[superscript app] and to describe their behavior as a function of pH under rapid equilibrium conditions. This approach provides students with an alternative way of understanding the effect of pH on enzyme kinetics that is complementary to the traditional derivations using pH-dependent kinetic equations.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A