ERIC Number: EJ1029334
Record Type: Journal
Publication Date: 2014
Abstractor: As Provided
Reference Count: N/A
An Undergraduate Study of Two Transcription Factors that Promote Lateral Root Formation
Bargmann, Bastiaan O. R.; Birnbaum, Kenneth D.; Brenner, Eric D.
Biochemistry and Molecular Biology Education, v42 n3 p237-245 May-Jun 2014
We present a lab that enables students to test the role of genes involved in the regulation of lateral roots growth in the model plant "Arabidopsis thaliana." Here, students design an experiment that follows the effects of the hormone auxin on the stimulation of genes involved in the formation of lateral root initials. These genes, known as lateral organ boundary domain containing protein ("LBD") genes, are upregulated in the presence of auxin as part of a multistep molecular and biochemically controlled pathway. Depending on which "LBD" gene is tested, and the stage of root development, expression patterns are localized in a discrete and punctate fashion at the site of lateral root initials ("LBD33"), or reveal a broader localization pattern ("LBD16"). Students view expression using the reporter gene "GUS" (beta-glucuronidase). Before GUS staining, students view root growth in a "pseudo-aseptic" agar-based environment that allows complete visualization of whole root development to determine the proper stage to test molecular expression.
Descriptors: Science Instruction, College Science, Genetics, Science Laboratories, Science Experiments, Scientific Concepts, Molecular Biology, Biochemistry, Laboratory Experiments
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Authoring Institution: N/A