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ERIC Number: EJ1029072
Record Type: Journal
Publication Date: 2014
Pages: 11
Abstractor: As Provided
Reference Count: N/A
ISSN: ISSN-1470-8175
Mapping a Mutation in "Caenorhabditis elegans" Using a Polymerase Chain Reaction-Based Approach
Myers, Edith M.
Biochemistry and Molecular Biology Education, v42 n3 p246-256 May-Jun 2014
Many single nucleotide polymorphisms (SNPs) have been identified within the "Caenorhabditis elegans" genome. SNPs present in the genomes of two isogenic "C. elegans" strains have been routinely used as a tool in forward genetics to map a mutation to a particular chromosome. This article describes a laboratory exercise in which undergraduate students use molecular biological techniques to map a mutation to a chromosome using a set of SNPs. Through this multi-week exercise, students perform genetic crosses, DNA extraction, polymerase chain reaction, restriction enzyme digests, agarose gel electrophoresis, and analysis of restriction fragment length polymorphisms. Students then analyze their results to deduce the chromosomal location of the mutation. Students also use bioinformatics websites to develop hypotheses that link the genotype to the phenotype.
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A