ERIC Number: EJ1025579
Record Type: Journal
Publication Date: 2013
Abstractor: As Provided
Reference Count: N/A
Environmental Regulation of Plant Gene Expression: An Rt-qPCR Laboratory Project for an Upper-Level Undergraduate Biochemistry or Molecular Biology Course
Eickelberg, Garrett J.; Fisher, Alison J.
Biochemistry and Molecular Biology Education, v41 n5 p325-333 Sep-Oct 2013
We present a novel laboratory project employing "real-time" RT-qPCR to measure the effect of environment on the expression of the "FLOWERING LOCUS C" gene, a key regulator of floral timing in "Arabidopsis thaliana" plants. The project requires four 3-hr laboratory sessions and is aimed at upper-level undergraduate students in biochemistry or molecular biology courses. The project provides students with hands-on experience with RT-qPCR, the current "gold standard" for gene expression analysis, including detailed data analysis using the common 2[superscript -??C[subscript T]] method. Moreover, it provides a convenient starting point for many inquiry-driven projects addressing diverse questions concerning ecological biochemistry, naturally occurring genetic variation, developmental biology, and the regulation of gene expression in nature.
Descriptors: Science Instruction, College Science, Genetics, Plants (Botany), Environmental Influences, Undergraduate Study, Biochemistry, Molecular Biology, Hands on Science, Science Laboratories, Scientific Concepts
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Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Authoring Institution: N/A