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ERIC Number: EJ1016953
Record Type: Journal
Publication Date: 2013-Dec
Pages: 12
Abstractor: As Provided
ISSN: ISSN-1043-4046
Glucose Transport into Everted Sacs of the Small Intestine of Mice
Hamilton, Kirk L.; Butt, A. Grant
Advances in Physiology Education, v37 n4 p415-426 Dec 2013
The Na[superscript +]-glucose cotransporter is a key transport protein that is responsible for absorbing Na[superscript +] and glucose from the luminal contents of the small intestine and reabsorption by the proximal straight tubule of the nephron. Robert K. Crane originally described the cellular model of absorption of Na[superscript +] and glucose by a "co transport process" in 1960. Over the past 50 plus yr, numerous groups have tested and verified Crane's hypothesis. Eventually, Wright and colleagues cloned the Na[superscript +]-glucose cotransporter (SGLT1; the product of the SLC5A1 gene) in 1987. This article provides a "hands-on" laboratory exercise using the everted mouse jejunal preparation (everted sac) that allows students to investigate various components of the Na[superscript +]-glucose cotransport absorptive cell model (e.g., Na[superscript +] dependence of SGLT1, inhibition of SGLT1, and inhibition of Na[superscript +]- K[superscript +]-ATPase). Additionally, the laboratory exercise includes a case-based study of glucose-galactose malabsorption in which the students conduct an internet search and participate in a small-group discussion during the laboratory period to better understand the basic principles and functions of the Na[superscript]-glucose absorptive process of the small intestine. This laboratory exercise was introduced into the second-year undergraduate physiology curriculum in 2008, and more than 850 physiology students have participated in this laboratory exercise. The students have produced very robust and reproducible data that clearly illustrate the theory of the cellular model for Na[superscript +]-glucose absorption by the jejunum.
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Identifiers - Location: New Zealand
Grant or Contract Numbers: N/A