ERIC Number: EJ1016947
Record Type: Journal
Publication Date: 2013-Dec
Abstractor: As Provided
Reference Count: 63
CO[subscript 2] Rebreathing: An Undergraduate Laboratory to Study the Chemical Control of Breathing
Domnik, N. J.; Turcotte, S. E.; Yuen, N. Y.; Iscoe, S.; Fisher, J. T.
Advances in Physiology Education, v37 n4 p361-369 Dec 2013
The Read CO[subscript]2 rebreathing method (Read DJ. "A clinical method for assessing the ventilatory response to carbon dioxide." "Australas Ann Med" 16: 20-32, 1967) provides a simple and reproducible approach for studying the chemical control of breathing. It has been widely used since the modifications made by Duffin and coworkers. Our use of a rebreathing laboratory to challenge undergraduate science students to investigate the control of breathing provided 8 yr of student-generated data for comparison with the literature. Students (age: 19-22 yr, Research Ethics Board approval) rebreathed from a bag containing 5% CO[subscript 2] and 95% O[subscript 2] (to suppress the peripheral chemoreflex to hypoxia). Rebreathing was performed, and ventilation measured, after hyperventilation to deplete tissue CO[subscript 2] stores and enable the detection of the central chemoreflex threshold. We analyzed 43 data sets, of which 10 were rejected for technical reasons. The mean threshold and ventilatory sensitivity to CO[subscript 2] were 43.3 plus or minus 3.8 mmHg and 4.60 plus or minus 3.04 l times min [superscript -1] times mmHg [superscript -1] (means plus or minus SD), respectively. Threshold values were normally distributed, whereas sensitivity was skewed to the left. Both mean values agreed well with those in the literature. We conclude that the modified rebreathing protocol is a robust method for undergraduate investigation of the chemical control of breathing.
Descriptors: Science Instruction, College Science, Science Laboratories, Chemistry, Physiology, Measurement Techniques, Hypothesis Testing, Foreign Countries, Regression (Statistics), Undergraduate Students, Laboratory Experiments
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Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Authoring Institution: N/A
Identifiers - Location: Canada