In recent years in tertiary institutions in Australia, there has been a large increase of enrolments in Education courses delivered via an online/external mode. This has raised a number of concerns around the nexus of theory and practice and whether pre-service teachers feel ready to teach after completing Education study online. The purpose of this study is to examine preservice teachers' perceived readiness to teach Health and Physical Education (HPE) after engaging with the subject fully in an online tertiary environment.Twenty-six pre-service teachers studying education online were involved in this study. Upon completion of the University semester and also after a practicum placement, qualitative data was collected detailing the pre-service teachers' perceptions in regard to their readiness to teach HPE. Pre-service teachers' perceptions are used as the primary data highlighting the varying levels of readiness to teach HPE.