The aim of this study was to determine the effect of dynamic software on prospective mathematics teachers' perception levels regarding information and communication technology (ICT). The study was conducted with senior prospective teachers studying in a department of secondary mathematics education. The data of the study used both quantitative and qualitative research approaches have been obtained using two different tests, namely "Technology Perception Scale" and "Computer Assisted Mathematics Instruction Perception Scale". Consequently, it has been observed in the study that learning how to use dynamic software positively affects prospective mathematics teachers' perception levels in a statistically significant way regarding the use of technology in education. In addition, at the end of the study, almost all prospective mathematics teachers were of the opinion that mathematical software will contribute to teaching activities, and they have added that such a contribution will manifest itself in visualization, concretization and result in more effective teaching. (Contains 8 tables and 4 figures.)