The purpose of this study is to assess the relationship between youth assets and neighborhood environmental variables and future no-tobacco use among youth; examining differences by gender. Five waves of annual data were collected from 1,111 youth randomly selected to participate in the Youth Asset Study (YAS). A marginal logistic regression model using all five waves of tobacco use, gender, and their interaction was used to compare the change in tobacco use over time between males and females. Individual assets, asset score total, the six neighborhood variables, and the environment composite score assessed at waves 1-4 were analyzed as time-varying and lagged while controlling for demographics to predict no-tobacco use at waves 2-5. Among 1,093 youth (Mean age=14.3; 53% female; 40% white, 28% Hispanic, 23% African American, 9% other), the percentage of youth smoking increased significantly from baseline to wave 5 (4 years after baseline) for both females and males. Among females, sixteen assets were prospectively related with no-tobacco use whereas among males, fourteen assets were prospectively related with no-tobacco use. There were no significant differences between genders, and the neighborhood environment overall did not have an impact in the relationship between youth assets and future no-tobacco use. (Contains 3 tables and 1 figure.)