The purpose of this study was to identify current practices in autism spectrum disorder (ASD) screening among early intervention and care providers in Indiana. Participants were asked about their ASD screening practices within the context of overall screening for developmental delays. Results indicated that providers conduct ASD screening less often than developmental screening, do not use formal screening tools when doing so, and feel unprepared to talk with families about concerns related to ASD. Providers reported that they feel inadequately prepared to perform ASD screening and are eager to receive training. Results will assist in identifying training needs of early intervention and care providers.