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ERIC Number: EJ1013642
Record Type: Journal
Publication Date: 2013-Apr
Pages: 4
Abstractor: As Provided
ISSN: ISSN-0021-9584
Liquid-Liquid Extraction of Insecticides from Juice: An Analytical Chemistry Laboratory Experiment
Radford, Samantha A.; Hunter, Ronald E., Jr.; Barr, Dana Boyd; Ryan, P. Barry
Journal of Chemical Education, v90 n4 p483-486 Apr 2013
A laboratory experiment was developed to target analytical chemistry students and to teach them about insecticides in food, sample extraction, and cleanup. Micro concentrations (sub-microgram/mL levels) of 12 insecticides spiked into apple juice samples are extracted using liquid-liquid extraction and cleaned up using either a primary-secondary amine (PSA) cartridge or a C[subscript 18] cartridge. Extracts are then separated by gas chromatography and analyzed with tandem mass spectrometry. The method has been successfully tested by three cohorts of quantitative analysis students to determine the concentration of the insecticides spiked at 50 ng/mL in apple juice. Students' average recoveries were between 78 and 121% using C[subscript 18] cartridges ("n" = 30). Using PSA cartridges, average recoveries were between 47 and 121% ("n" = 6). Overall, this laboratory experiment was successful in teaching analytical chemistry students about environmental exposure to insecticides and sample processing. (Contains 3 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A
Grant or Contract Numbers: N/A