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ERIC Number: EJ1012208
Record Type: Journal
Publication Date: 2013-Feb
Pages: 2
Abstractor: As Provided
Reference Count: 5
ISSN: ISSN-0021-9584
A Novel Active-Learning Protein Purification Exercise for Large-Enrollment Introductory Biochemistry Courses Using the CHROM Web Applet
Barrette-Ng, Isabelle H.; Usher, Ken C.
Journal of Chemical Education, v90 n2 p252-253 Feb 2013
The CHROM Web applet has been used to create a new active-learning exercise in which students design a purification scheme for a recombinant protein using ion-exchange chromatography (IEC). To successfully complete the exercise, students are challenged to apply elementary concepts from acid-base chemistry as well as protein and amino acid structure to devise a scheme purifying the target recombinant protein from other "Escherichia coli" proteins. By actively applying fundamental principles to solve an unfamiliar problem, students develop higher-level cognitive skills and gain a deeper understanding of key concepts that are difficult to teach effectively through traditional methods. The effectiveness of the exercise in promoting deep learning was assessed by comparing the performance of students in answering questions on acid-base chemistry, amino acid structure, and IEC before and after the completion of the active-learning exercise.
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A