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ERIC Number: EJ1012206
Record Type: Journal
Publication Date: 2013-Feb
Pages: 4
Abstractor: As Provided
Reference Count: 18
ISBN: N/A
ISSN: ISSN-0021-9584
Effect of pH on the Heat-Induced Denaturation and Renaturation of Green Fluorescent Protein: A Laboratory Experiment
Flores, Rosa V.; Sola, Hilda M.; Torres, Juan C.; Torres, Rafael E.; Guzman, Ernick E.
Journal of Chemical Education, v90 n2 p248-251 Feb 2013
A fluorescence spectroscopy experiment is described where students integrated biochemistry and instrumental analysis, while characterizing the green fluorescent protein excitation and emission spectra in terms of its phenolic and phenolate chromophores. Students studied the combined effect of pH and temperature on the protein's fluorescence, which led them to conclude that its fluorescence, denaturation, and renaturation was pH dependent. The importance of temperature control in fluorescence spectroscopy, and of control samples in experiments, was also stressed. This experiment is suitable for an upper-level biochemistry laboratory, as an extension of experiences involving bacterial transformation and hydrophobic interaction chromatography to purify the protein. It is also appropriate for an instrumental analysis laboratory using a provided protein sample. (Contains 3 tables and 2 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail: eic@jce.acs.org; Web site: http://pubs.acs.org/jchemeduc
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A