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ERIC Number: EJ1012182
Record Type: Journal
Publication Date: 2013-Feb
Pages: 7
Abstractor: As Provided
Reference Count: 34
ISSN: ISSN-0021-9584
Surface Plasmon Resonance Spectroscopy: A Versatile Technique in a Biochemist's Toolbox
Bakhtiar, Ray
Journal of Chemical Education, v90 n2 p203-209 Feb 2013
Surface plasmon resonance (SPR) spectroscopy is a powerful, label-free technique to monitor noncovalent molecular interactions in real time and in a noninvasive fashion. As a label-free assay, SPR does not require tags, dyes, or specialized reagents (e.g., enzymes-substrate complexes) to elicit a visible or a fluorescence signal. During the last two decades, SPR has been broadly applied to study of noncovalent interactions of protein-DNA, protein-cell, RNA-DNA, DNA-DNA, protein-protein, protein-carbohydrate, small molecule-macromolecule (e.g., receptor-inhibitor complex), protein-peptide, and self-assembled monolayers. In addition, SPR has been successfully applied to drug discovery ligand-fishing and clinical immunogenicity studies (i.e., to monitor an immune response against a therapeutic agent). SPR spectroscopy can address questions such as specificity of an interaction, kinetics, affinity, and concentrations of selected molecules present in a sample of interest. Given the current enhancements in hardware and software capabilities along with its ease of use and maintenance, SPR experiments can be designed for upper-level undergraduate biochemistry, biophysics, and physical chemistry laboratory courses. In this article, an overview of SPR phenomenon, instrumentation, sensor immobilization, and its selected applications is presented. (Contains 2 tables and 5 figures.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Research
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A