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ERIC Number: EJ1011424
Record Type: Journal
Publication Date: 2013-Jan
Pages: 3
Abstractor: As Provided
Reference Count: 10
ISSN: ISSN-0021-9584
Carbohydrate Analysis Experiment Involving Mono- and Disaccharides with a Twist of Glycobiology: Two New Tests for Distinguishing Pentoses and Glycosidic Bonds
Herdman, Chelsea; Diop, Lamine; Dickman, Michael
Journal of Chemical Education, v90 n1 p115-117 Jan 2013
Carbohydrate analysis is an excellent way to expose second-year biochemistry majors to the subtlety and nuance of sugar chemistry. In one, 3-h practical period, students must identify an unknown mono- or disaccharide from a collection of three hexoses (glucose, galactose, and mannose), two pentoses (ribose and arabinose), and three disaccharides (maltose, cellobiose, and lactose). Two new tests have been developed: an enzyme assay with "a"-glucosidase followed by the Barfoed test quickly distinguishes maltose and cellobiose and 1,1-diphenylhydrazine is used to identify arabinose. The exercise is presented as a way to sensitize new students of biochemistry to the communicative aspect of sugars. (Contains 1 figure.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A