Previous studies have shown that pouched rats can detect the presence of "Mycobacterium tuberculosis," which causes tuberculosis, in human sputum samples obtained from clinical facilities. Although pouched rats evaluate sputum samples quickly, preparing the samples is relatively slow. The present study evaluated whether the rats can detect tuberculosis in microscope slides, which are routinely prepared at these facilities. Results indicate that they can do so, but their accuracy is considerably lower than that of rats exposed to pots containing sputum, as in prior studies. (Contains 1 table and 2 figures.)