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ERIC Number: EJ1003087
Record Type: Journal
Publication Date: 2012-Nov
Pages: 4
Abstractor: As Provided
Reference Count: 12
ISSN: ISSN-0021-9584
A Discovery-Oriented Approach to Solid-Phase Peptide Synthesis
Bockman, Matthew R.; Miedema, Christopher J.; Brennan, Brian B.
Journal of Chemical Education, v89 n11 p1470-1473 Nov 2012
In this discovery-oriented laboratory experiment, students use solid-phase synthesis techniques to construct a dipeptide containing an unknown amino acid. Following synthesis and cleavage from the polymeric support, electrospray ionization-mass spectrometry is employed to identify the unknown amino acid that was used in the peptide coupling. This experiment, performed in two, 3-h laboratory periods, provides an opportunity for students to learn the technique of solid-phase peptide synthesis and to critically evaluate data that they generate in the laboratory. The experiment is suitable for second-semester organic chemistry students as they learn methods for the formation of amide bonds or upper-level biochemistry students as they learn about the primary structures of proteins. (Contains 1 figure and 4 schemes.)
Division of Chemical Education, Inc and ACS Publications Division of the American Chemical Society. 1155 Sixteenth Street NW, Washington, DC 20036. Tel: 800-227-5558; Tel: 202-872-4600; e-mail:; Web site:
Publication Type: Journal Articles; Reports - Descriptive
Education Level: Higher Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A