The present study comparatively examines the effects of culture on psychological mobility of academicians in Turkey and Canada. Questionnaires were completed by 382 respondents, of them 277 Turkish and 105 Canadian. Data were collected using INDCOL for measuring the four cultural dimensions. Psychological mobility that consisted of boundaryless mindset and organizational mobility preference was measured using the Boundaryless Career Attitude Scale. Findings of the study revealed that there were differences between Turkish and Canadian academicians in terms of four cultural dimensions and psychological mobility. It was also found that culture has influence on psychological mobility. Turkish academicians were more collectivist and had higher organizational mobility preference than Canadian counterparts.