Humor is a fundamental pedagogical tool that EFL teachers can utilize in the classroom to maximize their effectiveness. Furthermore, humor in pedagogy is a basic tool that can help teachers to create a motivating atmosphere in class to enhance student learning. For years, educational researchers have tried to investigate humor implementation in the classroom context and humor has been viewed as a classroom learning and teaching catalyzer. In this study, a questionnaire adapted from Morrison (2008), Askildson (2005), Pham (2014) was administered to 110 English preparatory program students at a developing state university located in the Aegean region of Turkey to collect data on their perceptions on teachers' using humor in the EFL classroom. The interpretation of the findings in this study indicated that the use of humor had positive effects (e.g. creating a more comfortable and conducive learning environment, making students feel more relaxed) in the classroom. All in all, the results showed that humor was important in the EFL classroom and that students indicated a high level of agreement on the positive role of humor in classroom teaching.