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ERIC Number: ED554874
Record Type: Non-Journal
Publication Date: 2013
Pages: 152
Abstractor: As Provided
ISBN: 978-1-3031-3378-7
Mesothelial Cell Autoantibodies Induce Collagen Deposition in vitro & Using a Case Study to Introduce Undergraduates to Bioinformatics
Serve, Kinta M.
ProQuest LLC, D.A. Dissertation, Idaho State University
Part I. Pleural fibrosis, a non-malignant, asbestos-related respiratory disease characterized by excessive collagen deposition, is progressive, debilitating, and potentially fatal. Disease severity may be influenced by the type of asbestos fiber inhaled, with Libby amphibole (LA) a seemingly more potent mediator of pleural fibrosis than chrysotile (CH) asbestos. This difference in severity may be due to the reported immunological component associated with LA but not CH related diseases. Here, we report the potential mechanisms by which asbestos-associated mesothelial cell autoantibodies (MCAAs) contribute to pleural fibrosis development. MCAAs are shown to bind cultured human pleural mesothelial cells and induce the deposition of type I collagen proteins in the absence of phenotypic changes typically associated with fibrosis development. However, additional extracellular proteins seem to differentially contribute to LA and CH MCAA-associated collagen deposition. Our data also suggest that IgG subclass distributions differ between LA and CH MCAAs, potentially altering the antibody effector functions. Differences in MCAA mechanisms of action and effector functions may help explain the disparate clinical disease phenotypes noted between LA and CH-exposed populations and may provide insights for development of novel therapeutic strategies. Part II. As scientific research becomes increasingly reliant on computational tools, it is more important than ever before to train students to use these tools. While educators agree that biology students should gain experience with bioinformatics, there exists no consensus as to how to integrate these concepts into the already demanding undergraduate curriculum. The Portal-21 project offers a solution by utilizing on-line learning case studies to allow flexibility for classroom integration. Presented here are the results from two field tests of a case study developed to introduce the common bioinformatics tools pBLAST and PubMed to undergraduate students while reinforcing concepts of protein function. Data suggest positive gains in student learning and confidence with using bioinformatics tools following use of the case study. These results indicate that on-line case studies are a useful tool for introducing bioinformatics into undergraduate classrooms. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page:]
ProQuest LLC. 789 East Eisenhower Parkway, P.O. Box 1346, Ann Arbor, MI 48106. Tel: 800-521-0600; Web site:
Publication Type: Dissertations/Theses - Doctoral Dissertations
Education Level: Higher Education; Postsecondary Education
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A