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ERIC Number: ED526657
Record Type: Non-Journal
Publication Date: 2009
Pages: 157
Abstractor: As Provided
Reference Count: 0
ISBN: ISBN-978-1-1095-7156-1
Dynamics of Agglutinin-Like Sequence (ALS) Protein Localization on the Surface of Candida Albicans
Coleman, David Andrew
ProQuest LLC, Ph.D. Dissertation, University of Illinois at Urbana-Champaign
The ALS gene family encodes large cell-surface glycoproteins associated with "C. albicans" pathogenesis. Als proteins are thought to act as adhesin molecules binding to host tissues. Wide variation in expression levels among the ALS genes exists and is related to cell morphology and environmental conditions. "ALS1," "ALS3," and "ALS4" are three of the four most highly expressed ALS genes. This work describes the production and use of specific high affinity monoclonal antibodies against Als1, Als3, and Als4 to evaluate protein production and localization at both the individual cell and population levels. Production and localization of these proteins is tightly regulated and occurs under specific growth conditions. When examined at a population level, it is clear that cells from the same culture can have different profiles of Als proteins on the cell surface. These results provide a very different view of the Als family than the view held by those who suggest that the Als family functions in antigenic variation in a classical sense. The monoclonal antibodies were also used to screen a diverse group of "C. albicans" strains and other "Candida" species for the presence of Als proteins or Als-like epitopes. Additionally, Als3 binding to several host proteins proposed to act as ligands for Als3 during "C. albicans" infection was characterized. Estimation of the number of Als1, Als3, and Als4 molecules on the cell surface allows evaluation of the potential role of Als3 binding to proposed host tissue ligands in "C. albicans" pathogenesis. [The dissertation citations contained here are published with the permission of ProQuest LLC. Further reproduction is prohibited without permission. Copies of dissertations may be obtained by Telephone (800) 1-800-521-0600. Web page:]
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Publication Type: Dissertations/Theses - Doctoral Dissertations
Education Level: N/A
Audience: N/A
Language: English
Sponsor: N/A
Authoring Institution: N/A