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ERIC Number: ED107634
Record Type: Non-Journal
Publication Date: N/A
Pages: 14
Abstractor: N/A
Reference Count: N/A
Effects of Fixatives and Buffers upon the Morphology of Heart and Skeletal Muscle Mitochondria from Exhausted Rats.
Gale, James B.
This study describes the effects of several fixatives and buffers on the morphology of mitochondria from resting and exhausted rats. Rats were run to exhaustion and adjacent portions from the left ventricle or from the soleus were treated with the following fixation procedures: (a) glutaraldehyde buffered with cacodylate, S-collidine, or phosphate and postfixed in osmium tetroxide; or (b) osmium tetroxide (as a primary fixative) buffered with cacodylate, veronal acetate, S-collidine, or phosphate or veronal acetate-Ringers with varying concentrations of Ca2+ ions (dicalcium ion with a plus-one charge). Mitochondria disruption was absent in all tissues prepared in buffered glutaraldehyde solutions. Mitochondria swelling, following exhaustive exercise, occurred in all of the buffered osmium solutions which did not contain Ca2+. It has been found that the degree of mitochondria swelling following exhaustive exercise was inversely related to the concentration of Ca2+. In this study, the presence of Ca2+ in fixatives for muscles from resting or only moderately exercised rats produced mitochondria swelling. (Figures describing fixative mixtures and their effects upon mitochondria are included.) (Author/JS)
Publication Type: Reports - Research
Education Level: N/A
Audience: N/A
Language: N/A
Sponsor: N/A
Authoring Institution: N/A