The non-profit sector is increasingly important, both in terms of the services rendered to society and level of employment provided. As part of a move towards the professionalization of the sector, training is seen as a vital tool for capacity building. Although the training practices of non-profits are fairly well documented in countries like Australia, the UK and the USA, this is not so in Portugal. We provide an overview of the training practices of Portuguese non-profit organizations, and identify indicators of professionalization that influence training investment. We find wide variation among organizations in this sector, both in terms of training provision and expenditure, and in the management practices implemented. We also find that the presence of a professional manager and formalized policies, especially those related to performance, are significantly associated with higher levels of training, suggesting that professionalization has beneficial effects on training in the non-profit sector.