The authors describe the development and initial validation of a home-based version of the Laboratory Temperament Assessment Battery (Lab-TAB), which was designed to assess childhood temperament with a comprehensive series of emotion-eliciting behavioral episodes. This article provides researchers with general guidelines for assessing specific behaviors using the Lab-TAB and for forming behavioral composites that correspond to commonly researched temperament dimensions. We used mother ratings and independent postvisit observer ratings to provide validity evidence in a community sample of 4.5-year-old children. 12 Lab-TAB behavioral episodes were employed, yielding 24 within-episode temperament components that collapsed into 9 higher level composites (Anger, Sadness, Fear, Shyness, Positive Expression, Approach, Active Engagement, Persistence, and Inhibitory Control). These dimensions of temperament are similar to those found in questionnaire-based assessments. Correlations among the 9 composites were low to moderate, suggesting relative independence. As expected, agreement between Lab-TAB measures and postvisit observer ratings was stronger than agreement between the Lab-TAB and mother questionnaire. However, for Active Engagement and Shyness, mother ratings did predict child behavior in the Lab-TAB quite well. Findings demonstrate the feasibility of emotion-eliciting temperament assessment methodologies, suggest appropriate methods for data aggregation into trait-level constructs and set some expectations for associations between Lab-TAB dimensions and the degree of cross-method convergence between the Lab-TAB and other commonly used temperament assessments. (Contains 1 figure, 9 tables and 1 footnote.)