Differences in the suicidal experiences of males and females and of undergraduate and graduate students have not been thoroughly explored. Furthermore, given the changing dynamics of college student suicidality and the challenges of suicide prevention, it is important to continue updating the research in this area. This article presents findings from a 2006 national survey with responses from over 26,000 undergraduate and graduate students at 70 colleges and universities. Gender and undergraduate versus graduate student differences were found in suicidal thoughts and behaviors, related experiences such as professional and informal help-seeking during a suicidal crisis, events that precipitated and contributed to the suicidal crisis, and factors that protected against attempting suicide. Among the many findings, some are consistent with previous research, some are unexpected, and some have not previously been studied. The results of this survey have implications for targeting specific interventions to better meet the needs of undergraduate and graduate student males and females. (Contains 5 tables.)