Purpose: The purpose of this study was to investigate inter- and intrarater reliability among expert users, novice users, and speech-language pathologists with a semiautomated high-resolution manometry analysis program. We hypothesized that all users would have high intrarater reliability and high interrater reliability. Method: Three expert users, 15 novice users, and 5 speech-language pathologists participated in this study. Following a 20-min training session, users analyzed 30 high-resolution manometry plots using an automated analysis program. Output parameters included 2- and 3-dimensional pressure integrals over 5 anatomical regions of interest. Intraclass correlations were used to examine inter- and intrarater reliability. An analysis of variance was also performed to determine any differences in mean output parameter values. Results: Within-group interrater reliability ranged from 0.54 to 0.99, and intergroup reliability ranged from 0.92 to 0.99. Intrarater reliability ranged from 0.67 to 1.00 across all groups. There were no significant differences of output parameters among groups. Conclusions: The high reliability observed after a short training session demonstrates that individuals with little to no prior knowledge of swallowing physiology can perform at a similar level as those with expertise. Given the quickness and ease of training in the use of this program, it has the potential for research and clinical utility.