Science 101 was designed by a multidisciplinary, multi-institutional team, with leadership from the Departments of Biology and Teacher Education, and participation by faculty in the Departments of Chemistry, Physics, and Mathematics, the College of Engineering, and master teachers from school districts in the state of Illinois. Their goal was to develop a course that would provide preservice teachers with the background necessary to teach inquiry-based, investigative science. This article describes the student learning outcomes and attitudinal shifts among the students enrolled in this course. (Contains 4 figures.)