The lecture content of a Plant Biology class for non-majors was transformed from a traditional on-campus lecture to an asynchronous website for use both as a stand-alone internet-based course and as an adjunct to the on-campus course. Additional information and examples of the online interactive activities included in PLB 108, Concepts in Plant Biology, can be found at http://is.asu.edu/plb108/ (verified 5 Mar. 2009). The 2-year planning and development process included a partnership between instructors in the Plant Biology Department and multimedia designers in the Instructional Technology Support Group of Arizona State University. The team developed, designed, and delivered a 259-page website rich in illustrations, images, animations, and videos. More than 100 interactive animations were used to illustrate complex processes and structures and for self assessments. (Contains 1 table and 2 figures.)