The present study aims at investigating which factors are significant for pupils' acceptance of an E-Learning system introduced into secondary schools. The E-Learning system consisted of interactive learning modules for several school subjects and a Learning Management System (LMS). Research on IT acceptance and attitudes guided the specification of independent variables as predictors of acceptance. Survey data were collected from 125 pupils from 8 German high schools in January and July 2005. Results from a three-step hierarchical regression reveal that perceived usefulness was a significant positive predictor of pupils' acceptance, with perceived usefulness of the LMS having the greatest weight. Computer related attitude, self-efficacy, and gender had negligible or no influence on acceptance. The final regression model accounted for approximately 51% of the variance in the outcome measure. The findings indicate the critical importance of perceived usefulness in predicting pupils' acceptance of the E-Learning system, as is likewise known from acceptance research on IT at the work place. (Contains 2 tables.)