The enzymatic resolution of "rac"-1-phenylethanol using ethyl myristate as acylating agent and solvent and "Candida antarctica" lipase B (CAL-B) as biocatalyst was demonstrated with catalyst and medium reuse. Both enantiomers of 1-phenylethanol were isolated by sequential enzymatic reactions and product distillations. From the first enzymatic reaction, ("S")-1-phenylethanol was isolated with a 41% yield and 80% enantiomeric excess, and from the second enzymatic reaction, ("R")-1-phenylethanol was isolated with a 56% yield and 63% enantiomeric excess. (Contains 2 figures and 1 note.)