We developed a modular pair of experiments for use in the undergraduate physical chemistry and biochemistry laboratories. Both experiments examine the thermodynamics of the binding of a small molecule, eosin Y, to the protein lysozyme. The assay for binding is the quenching of lysozyme fluorescence by eosin through resonant energy transfer. In…

We present a multiweek laboratory exercise that exposes students to the fundamental techniques of bacterial expression and protein purification through the preparation of sperm whale myoglobin. Myoglobin, a robust oxygen-binding protein, contains a single heme that gives the protein a reddish color, making it an ideal subject for the teaching…

In this activity, students use a model created from a coffee cup or cardstock cutout to explore the working principle of an atomic force microscope (AFM). Students manipulate a model of an AFM, using it to examine various objects to retrieve topographic data and then graph and interpret results. The students observe that movement of the AFM…

A first-year laboratory experiment that utilizes concepts of electrochemical tip etching for scanning tunneling microscopy (STM) is described. This experiment can be used in conjunction with any STM experiment. Students electrochemically etch gold STM tips using a time-efficient method, which can then be used in an instructional grade STM that…

We have developed a two-week guided-inquiry laboratory in which first-semester general chemistry students investigate a suspected arson using gas chromatography--mass spectrometry and paper chromatography. In the process of evaluating evidence from the crime scene, students develop and test hypotheses and learn the fundamentals of chromatography,…

Static headspace analysis of accelerants from suspected arsons is accomplished by placing an arson sample in a sealed container with a carbon strip suspended above the sample. The sample is heated, cooled to room temperature, and then the organic components are extracted from the carbon strip with carbon disulfide followed by gas chromatography…

In analytical chemistry classes, the importance of gathering a proper sample for analysis of bulk materials is often addressed only briefly or not at all. Although a number of classroom and laboratory exercises have been developed to illustrate factors that impact error introduced by sampling, they generally do not demonstrate the main goal of…

There are relatively few experiments describing the NMR properties of bis(amine) iron(II) phthalocyanine complexes. Several features make this experiment attractive: First, it nicely illustrates the diamagnetic anisotropy phenomena, providing both students and teachers an opportunity to gain insight into aspects such as phase correction and…

A new laboratory procedure for the evaluation of the mean molecular weight (mean relative molecular mass) of petroleum oils with high accuracy is described. The density and dynamic viscosity of three commercial petroleum oils are measured at different temperatures. These experimental data are used to calculate the kinematic viscosity as a function…

We describe the construction and performance of an inexpensive modular Raman spectrometer that has been assembled in the framework of a fourth-year undergraduate project (costs below $5000). The spectrometer is based on a 4 mW 532 nm green laser pointer and a compact monochromator equipped with glass fiber optical connections, linear detector…

Most thermodynamic properties are either extensive (e.g., volume, energy, entropy, amount, etc.) or intensive (e.g., temperature, pressure, chemical potential, mole fraction, etc.). By the same token most of the mathematical relationships in thermodynamics can be written in extensive or intensive form. The basic laws of thermodynamics are usually…

This paper examines quantitative differences among groups of first-year science education students regarding student- or teacher-centeredness while teaching. Our research sample comprised four groups of first-year students from university-level teacher-training programs for both primary school science and for secondary school in the subjects of…

Chemical information instruction (CII) has been recommended by the ACS Committee on Professional Training as a necessary component of the chemistry curriculum for both undergraduate and graduate students. Surveys conducted by the ACS Chemical Information Division (CINF) Education Committee in 1984 and 1993 showed the extent that CII had become…

This paper offers an overview of the development of the peer-led team learning (PLTL) model for teaching and learning chemistry, from the personal journeys of the authors in their classrooms to the national dissemination of the model to the full range of colleges and universities and to other STEM disciplines. In the PLTL model, students who have…

The statistical picture of the gender composition of chemistry as reported in national data indicates that women are underrepresented in academe in comparison to their representation in the field as a whole. This article presents data on the perceptions and views of a broad cross-section of women in academic chemistry departments and provides some…